中国临床康复2005，Vol.9Issue(1)：196-197,2.
酸性肽对血管性痴呆模型小鼠的治疗效应
Therapeutic effects of acidic peptide on mouse model of vascular dementia
摘要
Abstract
BACKGROUND: If it can prove the therapeutic effects of acidic peptide when applying it to the mouse model of vascular dementia(VD).OBJECTIVE: To study the memory ability, change of contents of protein and nitric oxide of mouse model of VD and make comparison with normal mice.DESIGN: A randomized and experimental study.SETTING: Biochemistry & Molecular Biology Teaching Section of College of Medicine, Zhengzhou University.PARTICIPANTS: The experiment was completed in the First Research Unit and Animal Centre of Biochemistry & Molecular Biology Teaching Section of Zhengzhou University. Totally 84 mice of Kunming species were selected and the mouse was excluded because its average response time was slower than normal mouse when performing step-down avoidance test. The mice were randomly divided into 7 groups: normal group, model group, saline group,naofukang group, high dose acidic peptide group, moderate dose acidic peptide group and low dose acidic peptide group.METHODS: All the mice excepts of 12 in normal group were conducted stomach perfusion of high fat emulsion 0.5 mL per day for 10 days to establish VD animal model according to the method of Gao et al. There was no treatment for model group. While saline, acidic peptide high, moderate and low dose of acidic peptide was orally administrated to saline group, naofukang group, high dose, moderate dose and low dose acidic peptide group respectively for 15days. Then learning and memory functions were tested by step-down avoidance test. After being executed, protein and nitric oxide in mouse brain was assayed.MAIN OUTCOME MEASURES: Learning and memory ability of mice in each group as well as the content of protein and nitric oxide in brain.peptide had greatly reduced the mistaken times in mice and dramatically chemical analysis showed that the content of protein in mice of model group [ (3. 530 9 ± 0. 327 8) g/L] was reduced comparing with mice in normal group [ (4. 028 3 ± 0. 378 3) g/L] . After administrating acidic peptide to dementia mice, the protein content in high, moderate and low dose group was (4. 908 3 ± 0. 515 3) g/L, (4. 752 5 ± 0. 471 7) g/L and(4. 376 7 ±0. 370 7) g/L respectively which was greatly increased when compared with model group and saline group. It suggested that acidic peptide could stimulate the synthesis of protein in brain, which was good for the resynthesis of nitric oxide and reduced the toxic effects of NO to brain cells.CONCLUSION: Acidic peptide can improve the ability of learning and memory of mice with VD greatly and also has good effects on the plerosis of injured cerebral cells and the rehabilitation of its function.关键词
痴呆,血管性/疾病模型,动物/一氧化氮分类
医药卫生引用本文复制引用
安玉会,蔡尚党,崔福爱,赵荷键..酸性肽对血管性痴呆模型小鼠的治疗效应[J].中国临床康复,2005,9(1):196-197,2.基金项目
河南省杰出人才基金(021000500) （021000500）
