摘要
Abstract
BACKGROUND: There are many studies on the association between the insertion/deletion (I/D) polymorphism of angiotensin Ⅰ converting enzyme(ACE) gene and human velocity endurance at home and abroad, but differences in subjects, such as race difference, methods, condition resulted in different findings. 800-meter race is a better test to reflect human velocity endurance. OBJECTIVE: To investigate the association between the I/D polymorphism in the ACE gene and human velocity endurance.DESIGN: Controlled observation.SETTING: Center for Physical Ability, Scientific Research Institute, Chinese PLA Institute of Physical Education. PARTICIPANTS: Forty health male subjects of Han population, aged (21±2) years, were enrolled in the study at the Chinese PLA Institute of Physical Education from June to July 2004. They had similar sports history, and no history of cardiovascular diseases and diabetes mellitus.METHODS: 3 mL blood was extracted from subjects' peripheral veins to detect the I/D polymorphism in ACE gene by using polymerase chain reaction(PCR) technique. The results of 800-meter race were recorded, and the concentration of blood lactic acid was measured before and after 800-meter race with YSI 1 500 SPORT Lactate Analyzer. All the subjects were divided into high-velocity endurance group and low-velocity endurance group according to the results of 800-meter race.MAIN OUTCOME MEASURES: Detection of I/D genotype, result of 800-meter race, and concentration of blood lactic acid.RESULTS: Forty subjects were enrolled and involved in the result analysis. ①There were three I/D genotypes: Ⅱ(490 bp), DD(190 bp), ID(190,490 bp). ②According to the running speed in 800-meter race, 40 subjects were classified into three types: the first 18 subjects were considered astype 1, the last one as type 3, and others as type 2. Analysis of variancede monstrated that the probability of distance difference between every two types was all 0.000, indicating a better clustering effect. Eighteen subjects of type 1 were assigned to the high-velocity endurance group, and other 22 ones of types 2 and 3 were assigned to the low-velocity endurance group. ③No significant differences were found in the distributions of ACE genotypes and ACE allele between the two groups (P ＞ 0.05). ④At the end of 800-meter race, concentration of blood lactic acid was increased significantly from (2.17±0.56) mmol/L to (14.93±2.46) mmol/L in population of Ⅱ genotype, from (2.41±0.70) mmol/L to(14.86±1.54) mmol/L in those of ID genotype,and from (1.99±0.27) mmol/L to(14.13±1.51) mmol/L in those of DD genotype. And there were significant differences (P ＜ 0.01).However, whether at quiet state, after 800 m run or the ascending value after 8 000 m run, the concentrations of the blood lactic acid did not exist significant difference among the three kinds of genotypes (P ＞ 0.05).CONCLUSION: There is no association between the I/D polymorphism of ACE gene and human velocity endurance.关键词
肽基二肽酶A/多态现象,遗传/身体耐力分类
医药卫生
