南京医科大学学报（英文版）2003，Vol.17Issue(4)：196-200,5.
载脂蛋白E基因多态性与血脂代谢的关系
The Effects of Apolipoprotein E Polymorphism on Serum Lipids, Lipoproteins and Apolipoproteins Variation
摘要
Abstract
Objective: To study the effects of Apolipoprotein E ( ApoE) polymorphism on serum levels of lipids, lipoproteins and apolipoproteins. Methods: Fragments of ApoE gene forth ex-on containing codon 112 and 158 polymorphic locus were amplified by PCR, and then digested with CfoI endonuclease. Genotypes and alleles frequencies of 168 healthy persons in Jiangsu area were calculat-ed. The effects of ApoE genotypes and alleles on serum lipids, lipoproteins and apolipoproteins varia-tion were analyzed. Results: The effects of ApoE alleles on total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), ApoB was: along a decreasing gradient ε4＞ε3＞ε2. The effect of ε4 allele was to increase serum levels of TC, LDL-C and ApoB, and the ε2 allele had an effect oppositeto that of ε4 allele. Conclusion: ApoE polymorphism is an independent genetic factor on individualserum levels of lipids and apolipoproteins.关键词
脱辅基脂蛋白类/多态性/聚合酶链反应/限制性片段长度多态性Key words
Apolipoprotein E/polymorphism/polymerase chain reaction/restric-tion fragment length polymorphism分类
医药卫生引用本文复制引用
潘闽,朱健华,袁瑾,王惠民,刘志华..载脂蛋白E基因多态性与血脂代谢的关系[J].南京医科大学学报（英文版）,2003,17(4):196-200,5.基金项目
Supported by the Natural ScienceFoundation of Jiangsu Province (BK99160) and the Foundationof Nantong Science and Technology Committee (S9903) （BK99160）
