中国动脉硬化杂志2009，Vol.17Issue(3)：197-201,5.
RAW264.7细胞泡沫化前后差异蛋白质组分析
Differential Proteomics Research in the RAW264.7 and Foam Cells Induced from RAW264.7
沈驰斌 1卢德赵 1沃兴德 1李晓娟 1江婷婷 1王科1
作者信息
- 1. 浙江中医药大学生命科学学院,浙江省杭州市,310053
- 折叠
摘要
Abstract
Aim Two-dimensional difference gel electrophoresis (2-D DIGE) and mass spectrum were used to analyse the differential proteins between RAW264.7 cells and foam cells. Methods RAW264.7 cells cultured in vitro was transformed to foam cells by treating with ox-LDL. The total proteins of the RAW264.7 cells and foam cells were extracted and labeled with Cy3 and Cy5. Each Cy3-labeled sample and Cy5-labeled sample were mixed on the same 2-D gel along with a Cy2-1abeled mixture of all samples as an internal standard and run on the same gel. All images were analyzed by DeCyder software DIGE technology with internal standard. 12 unique proteins were selected, and in which 10 proteins were successfully identified. Results The expression of Stress protein 70, Disulfide bond isomerase and Cy-toplasm actin were decreased in foam cells, then the expression of Glucose regulated protein, Enol protein, Peroxiredoxin 4, Stathmin 1 and BID protein were increased. Conclusions The proteomics maps of RAW264.7 cells and foam cells were established, and some different proteins were selected and identified. Molecular mechanisms about the formation of foam cells was explored from proteomics-level, and provided new routes and new targets for the intervention study of foam cells transformation to atheromatous plaque.关键词
泡沫细胞/RAW264.7细胞/蛋白质组Key words
Foam Cells /RAW264.7 Cells/Proteomics分类
生物科学引用本文复制引用
沈驰斌,卢德赵,沃兴德,李晓娟,江婷婷,王科..RAW264.7细胞泡沫化前后差异蛋白质组分析[J].中国动脉硬化杂志,2009,17(3):197-201,5.
