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高效液相色谱-脉冲安培检测法测定脑微透析溶液中单胺类神经递质

徐桂芸 常溪燕 常理文

分析化学2001,Vol.29Issue(2):199-201,3.
分析化学2001,Vol.29Issue(2):199-201,3.

高效液相色谱-脉冲安培检测法测定脑微透析溶液中单胺类神经递质

Determination of Monoamine Neurotransmitters and Related Compounds in Microdialysis Solution by High Performace Liquid Chromatography with Pulsed Amperometric Detection

徐桂芸 1常溪燕 1常理文1

作者信息

  • 1. 中国科学院化学研究所,分子科学中心,
  • 折叠

摘要

Abstract

A method for determinattion of monoamine neurotransmitters and related compounds in microdialysis solution by high performance liquid chromatography (HPLC) with pulsed amperometric detection was described. A comparison of response of iso potential anperometric (AD) and pulsed amperometric detection (PAD) was made. The results indicated that the sensitivity of PAD was 1.2 fold that of AD. The quantitative determination of monoamine neurotransmitters and related compounds was carried out with 3,4-dihydroxybenzylamine (DHBA) as internal standerd. The recoveries of monoamine neurotransmitters in microdialysis solution are 71% ~ 101%.

关键词

神经递质/脉冲安培检测/高效液相色谱

分类

化学化工

引用本文复制引用

徐桂芸,常溪燕,常理文..高效液相色谱-脉冲安培检测法测定脑微透析溶液中单胺类神经递质[J].分析化学,2001,29(2):199-201,3.

基金项目

本文系国家自然科学基金资助项目。 ()

分析化学

OA北大核心CSCDSCI

0253-3820

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