中国临床药学杂志2007，Vol.16Issue(4)：199-203,5.
更昔洛韦滴眼液在家兔房水内的药物浓度测定及其药动学特征
Determination of ganciclovir in rabbit aqueous humor and pharmacokinetics of its eyedrops
沈雁 1涂家生 1陆洋 1林琳1
作者信息
- 1. 中国药科大学药剂学教研室,南京,210009
- 折叠
摘要
Abstract
AIM To develop an analytical HPLC method for the determination of ganciclovir concentration in rabbit aqueous humor and to study the ocular pharmacokineties of its eyedrops.METHODS Proteinaceous material in rabbit aqueous humor was precipitated by 20% (V/V) perchloric acid.An aliquot of the supernatant Was analyzed by HPLC.The chromatography Was performed on a reversed-phase encapped column (LiChrospher C18) at 30℃ with a mobile phase pharmacokinetic studies.The average recoveries were determined as 97.0%,98.7%,and 102.4% at a concentration in-day and inter-day coefficient of variation Was lower than 5%.To study its pharmacokinetic bioavailability.the eyedrop formulation of ganciclovir was given to 40 healthy rabbits.According to 3P87 program,the concentration-time curve was fit for two-compartment open model and the major parameters were as follows:t1/2α(1.95±0.78)h,t1/2β(26.46±This method is a selective,sensitive and reproducible method for the determination of the ganciclovir in rabbit aqueous humor and is suitable for the pharamacokinetic study of ganciclovir in ophthalmic system.关键词
更昔洛韦/高效液相色谱/家兔房水/滴眼液/药动学Key words
ganciclovir/HPLC/rabbit aqueous humor/eyedrops/pharmacokinetics分类
医药卫生引用本文复制引用
沈雁,涂家生,陆洋,林琳..更昔洛韦滴眼液在家兔房水内的药物浓度测定及其药动学特征[J].中国临床药学杂志,2007,16(4):199-203,5.
