江西农业大学学报2001，Vol.23Issue(2)：200-202,3.
类番茄茄茎段原生质体再生成株的研究
Plant Regeneration from Stem－Derived Protoplasts of
Solanum lycopersicoides
张长远 1吴定华2
作者信息
- 1. 广东省农业科学院蔬菜研究所,
- 2. 华南农业大学园艺系,
- 折叠
摘要
Abstract
A procedure for protoplast isolation, culture and plant regeneration has been developed for solanum lycopersicoides. Stem－protoplasts were diluted to 1×105/mL and cultured in HMA medium, cell colonies (20～30 cells) appeared after 10 days, micro calli (1～1.5mm) formed after 38～40 days from initially culturing. The micro calli were transferred to MC greenig medium for 2 weeks. The green calli were transferred to 15# medium for shoot inducting. The shoots which excised from the callus were transferred to test tubes with 50p medium first for root promoting about 3～4 days and then transferred to 50# rooting medium. Plants regenerated after 80～90 days after initially culturing. All the plants which potted in soil could vigorously grow and blossom.关键词
类番茄/茎段原生质体/再生植株分类
农业科技引用本文复制引用
张长远,吴定华..类番茄茄茎段原生质体再生成株的研究[J].江西农业大学学报,2001,23(2):200-202,3.
