中德临床肿瘤学杂志（英文版）2006，Vol.5Issue(3)：200-203,4.
MTA1基因表达与人骨肉瘤细胞浸润和转移的关系
Relationship between the Expression of MTA1 Gene and Invasion and Metastasis of Human Osteosarcoma Cells
摘要
Abstract
Objective: To compare the expression level of metastasis associated-1 (MTA1) in the higher and lower metastasis sublines of human osteosarcoma cells (MG63), and to investigate the relationship between the expression level of MTA1-EGFP and in vitro invasion and metastasis of human osteosarcoma cells.Methods: The expression level of MTA1 in two sublines of MG63 cells was detected by semi-quantitative RT-PCR, and cell invasion assay and cell proliferation assay were used to evaluate the invasive capacity in vitro in two sublines. The lower metastasis line of MG-63 cells were transfected with MTA1-EGFP full-length cDNA expression plasmid by lipofectamine. The changes of the MTA1-EGFP expression and in vitro invasion potential were measured after transfection. Results: M8 subline expressed significantly higher level of MTA1 than that of M6 subline by RT-PCR. The invasive potentials of low metastasis MG63cell line were increased after MTA1 gene transfection. Conclusion: There may be a relationship between MTA1 and invasive potentials of human osteosarcoma cells, and MTA1 may play a role in the molecular mechanism of tumor metastases and be a potential target for gene therapy of osteosarcoma. Further studies of MTA1 in human ostersarcoma cell metastasis are needed.关键词
肿瘤转移相关基因1/骨肉瘤/浸润/转移Key words
metastasis associated-1/osteosarcoma/invasion/neoplasm metastasis分类
医药卫生引用本文复制引用
李新志,陈安民,易成腊,郭风劲,罗政强,曾恒,徐卫国..MTA1基因表达与人骨肉瘤细胞浸润和转移的关系[J].中德临床肿瘤学杂志（英文版）,2006,5(3):200-203,4.基金项目
This project was supported by 973 National Great Foundation Research Program of China (No. 2002CB513107). （No. 2002CB513107）
