南京医科大学学报(英文版)2003，Vol.17Issue(5)：201-207,214,8.
HIV-1感染相关细胞因子激活BC-3细胞中潜伏感染的人类疱疹病毒8型的复制研究
Reactivation of Latent Infection of Human Herpesvirus 8 in BC-3 Cells from Primary Effusion Lymphoma by Recombinant Cytokines Similar to that Produced by HIV-1-infected T Cells
摘要
Abstract
Objective: To study and confirm that recombinant cytokines similar to those produced by HIV-1 infected T cells induced lytic cycle replication of human herpesvirus 8 (HHV-8) in BC-3 cells, another cell line from primary effusion lymphoma (PEL). Methods: The persistent stimulation of BC-3 was conducted by several cytokines known to be produced by HIV-1-infected T cells and important in growth and proliferation of Kaposi's sarcoma(KS)cells in vitro, such as the interferonγ (IFN-γ), the hepatoeyte growth factor/scatter factor (HGF/SF), the Oncostain M (OSM), and the tumor necrosis factor-a (TNF-a)which is not produced by HIV-1-infected T cells. Treated and un treated BC-3 cells were collected at the 3rd and 7th day of persistent stimulation, respectively. Immunohistochemical (IHC) staining, Northern blot, quantitative PCR (real-time PCR) and electron microscopy (EM) were carried out to detect the expresswn of immunogenic protein ORF59, messenger RNA (mRNA) of minor capsid protein ORF26, and the presence of viral particles of HHV-8 from treated and untreated BC-3 cells. Results: It showed that IFN-γ, HGF/SF, OSM, and TNF-a were found to induce an increase in mRNA expression of ORF26 when added individually to BC-3 cells. Particularly, ORF26 expression stimulated with IFN-γ and TNF-a respectively, increased 6. 1 and 2. 5-fold(from real-time PCR results)at the 7th day when compared with untreated BC-3 cells.Meanwhile, about 20% of IFN-γ stimulated BC-3 cells expressed ORF 5 9 at the 7th day as compared with 1. 5 % of untreated BC-3 cells when IHC staining was employed. In addition, viral particles of HHV-8 were readily identified in BC-3 cells stimulated with IFN-γ at the 7th day with EM analysis.Conclusion: TNF-a and recombinant cytokines beingsimilar to those produced by HIV-1 infected T Cellscould really induce HHV-8 lytic cycle replication inBC-3 cells, another cell line of PEL.关键词
人类疱疹病毒8型(HHV-8)/细胞因子/可溶性周期复制Key words
human herpesvirus 8 (HHV-8)/cytokines/lytic cycle replication分类
医药卫生引用本文复制引用
卢春,曾怡,黄丽..HIV-1感染相关细胞因子激活BC-3细胞中潜伏感染的人类疱疹病毒8型的复制研究[J].南京医科大学学报(英文版),2003,17(5):201-207,214,8.基金项目
Supported by Grant from the National Natural Science Foundation of China (30100160,30271179) （30100160,30271179）
