中国临床康复2006，Vol.10Issue(44)：202-203,2.
APP17肽改善糖尿病小鼠脑组织Tau蛋白过度磷酸化
Improved effect of APP17 peptide on overexpression of phosphorylated Tau protein in brain tissues of mice with diabetes mellitus
摘要
Abstract
BACKGROUND: Overexpression of phosphorylated Tau protein is a factor of dementia, and scholars abroad find that APP17 peptide may have effect on it.OBJECTIVE: To observe changes of phosphorylated Tau protein Ser202/Thr205 of mice with diabetes mellitus (DM) after injection of APP17 peptide.DESIGN: Randomized control study.SETTING: Department of Pathology, Capital University of Medical Sciences; Department of Brain Aging, Xuanwu Hospital, Capital University of Medical Sciences.MATERIALS: The experiment was carried out in the Pathological Department of Capital University of Medical Sciences and Brain Aging Department of Beijing Xuanwu Hospital. A total of 18 male Kunming mice of 8 weeks old and weighing 28-32 g were randomly divided into control group, DM group and APP17 peptide group with 6 in each group.METHODS: DM models were induced by streptozotocin (STZ) through selectively destroying β-islet cells; meanwhile, APP17 peptide was intraperitoneally injected into mice. Four weeks later, brain tissue underwentimmunohistochemical staining with AT-8 (Ser202/Thr205, a special monoclonal antibody).MAIN OUTCOME MEASURES: ① Morphological observation; ② AT-8 distribution; ③ quantitative analysis of immunohistochemical staining.RESULTS: Positive AT-8 cells in DM group were distributed in retrosplenial cortex, hippocampus, thalamus, hypothalamus, etc.; however, those incontrol and APP17 peptide groups were only distributed in retrosplenial cortex and hippocampus, and poorly stained.CONCLUSION: Positive AT-8 cells may be widely distributed in neurons of brains of DM mice; however, APP17 peptide may normalize the expression of positive AT-8 cells.关键词
糖尿病/Tau蛋白质类/淀粉样β蛋白前体分类
医药卫生引用本文复制引用
王蓬文,陆珊,雷亚平,赵志炜,姬志娟,盛树力..APP17肽改善糖尿病小鼠脑组织Tau蛋白过度磷酸化[J].中国临床康复,2006,10(44):202-203,2.基金项目
国家科技部"九七三"课题资助项目(G2000057010)Key Basic Research and Development of National 973 Program, No. G2000057010 （G2000057010）
