中华医学杂志（英文版）2004，Vol.117Issue(2)：202-206,5.
Telomerase activity in cervical intraepithelial neoplasia
Telomerase activity in cervical intraepithelial neoplasia
王淑珍 1孙建衡 2张伟 3金顺钱 3王洪平 3金玉生 3曲萍 3刘毅 3李茉3
作者信息
- 1. Department of Obstetrics and Gynecology, Chaoyang Hospital,Capital University of Medical Sciences, Beijing 100020, China
- 2. Department of Gynecological Oncology,Cancer Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China
- 3. Department of Centre for Tumor Assay,Cancer Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China
- 折叠
摘要
Abstract
Background It was reported that telomerase expression is closely associated with cellular immortality and cancer. This study was designed to investigate the relationship between telomerase expression and the carcinogenesis of cervical cancer, the possible use of telomerase as a marker of cervical intraepithelial neoplasia (CIN) progression or regression, and the natural history of CIN. Methods Telomeric repeat amplification protocol (TRAP) assay was used to measure telomerase activity in cervical scrapings and biopsy samples obtained from 105 cases affected with various cervical conditions, including chronic cervicitis (n=20), CIN (n=64, 16 cases of CIN Ⅰ , 20 cases of CIN Ⅱ, and 28 cases of CIN Ⅲ ), and invasive squamous cell carcinoma (n =21 ).Results In exfoliated cell samples, telomerase activity was detected in 5 of 20 (25. 0% ) cases of cervicitis, 10 of 16 (62.5%) cases of CIN Ⅰ , 11 of 20 (55.0%) cases of CIN Ⅱ, 23 of 28 (82.1%) cases of CIN Ⅲ, and 13 of 21 (61.9%) cases of carcinoma. In cervical biopsy samples, telomerase activity was detected in 6 of 20 (30. 0%) cases of cervicitis, 8 of 16 (50. 0%) cases of CIN Ⅰ , 9 of 20 (45.0%) cases of (CIN Ⅱ, 27 of 28 (96. 4%) cases of CIN Ⅲ, and 20 of 21 (95. 2%) cases of carcinoma. Telomerase activation was significantly higher in CIN samples than in cervicitis samples. Telomerase activity was detected at similar frequency in samples from cervical scrapings and cervical biopsies.Conclusion These results seem to suggest that telomerase expression may be associated with carcinogenesis of the cervix. TRAP assay of cervical scraping samples could be used to monitor and predict the development of CIN in clinical practice.关键词
cervical intraepithelial neoplasia/telomerase/enzymology/cervix neoplasmKey words
cervical intraepithelial neoplasia/telomerase/enzymology/cervix neoplasm分类
医药卫生引用本文复制引用
王淑珍,孙建衡,张伟,金顺钱,王洪平,金玉生,曲萍,刘毅,李茉..Telomerase activity in cervical intraepithelial neoplasia[J].中华医学杂志（英文版）,2004,117(2):202-206,5.
