南京林业大学学报（自然科学版）2005，Vol.29Issue(1)：20-24,5.
AFLP分子标记在鉴定海滨锦葵杂交后代上的应用
Identification of Hybrids of Kosteletzkya virginica Using AFLP Markers
摘要
Abstract
Kosteletzkya virginica is a potential halophytic grain crop for consumption and industrial utilization. AFLP markers were used to identify 36 F1 hybrids of two parents. Eighteen peimer combinatios amplified 1100 AFLP band between parent Hy103and Hh182,108(9.82%)of which were polymorphic. Total 1182AFLPP bands were amplified between parent Slhy353 and Blho301 ,148(12.51%) of which were polymorphic. primer combination E+AAC/M+CTC amplified five paternal characteristic bands between Hy103 and Hh182:401bp,390bp,156bp,137bpand 108bp,whereas E+AAC/M+CTT generated seven paternal characteristic bands :457bp,356bp,312bp,251bp,190and 176bp. All these paternal characteristic bands were found in eight F1bhybrids of Hy103×Hh182 ,which were real hybrid .Primer combinatoin E+AAC/M+CTT amplified five paternal characteristic bands between Slhy353 and Blho301:440bp, 393bp,289bp,162bpand 67bp, whereas E+ACA/M+CAT kgenerated four characteristic bands :448bp,360bp,217bpand 145bp.All these paternal characteristic four characteristic bands:448bp,360bp,217bpand 145bp.All these paternal characteristic bands were found in 26of 28F1 hybrids of slhy353×Blho301,and other two F1 hybries were false hybrids.关键词
海滨锦葵/AFLP/杂种/分子鉴定Key words
Kosteletzkya virginica/AFLP/Hybrid/Molecular identification分类
农业科技引用本文复制引用
阮成江,钦佩,韩睿明,何祯祥..AFLP分子标记在鉴定海滨锦葵杂交后代上的应用[J].南京林业大学学报（自然科学版）,2005,29(1):20-24,5.基金项目
国家高技术研究发展计划(863计划)资助项目(2002AA629210) （863计划）
