中国林学(英文版)2003，Vol.5Issue(1)：20-24,5.
Comparison of G6PDH Activity and LT50 Between P. tomentosa and P.suaveolens During Freezing Acclimation
Comparison of G6PDH Activity and LT50 Between P. tomentosa and P.suaveolens During Freezing Acclimation
摘要
Abstract
The differences of glucose-6P dehydrogenase (G6PDH) activity and freezing resistance induced by freezing acclima-tion between cuttings of freezing-sensitive P. tomentosa and freezing-resistant P. suaveolens were compared for exploring the role ofG6PDH on the enhancement of freezing resistance induced by freezing acclimation. After 5 d of freezing acclimation at -3 ℃, thcLT50 of P. tomentosa has decreased from -6.2 ℃ in control cuttings to -14.3 ℃ in freezing acclimated ones, and the increase ofG6PDH activity was observed in freezing acclimated cuttings as compared with control ones. Whereas, when P. suaveolens wasfreezing acclimated at -20℃ for 5 d, the LT50 has decreased from -27. 1℃ in control cuttings to -43.5 ℃ in freezing acclimated ones,and the activity of G6PDH increased considerably. In addition, the increase of LT50 and the decrease of G6PDH activity resultingfrom 2 d of deacclimation at 25 ℃ were found in two kinds of freezing acclimated cuttings. It is concluded that the increase in theactivity of G6PDH may associate with the inherited freezing resistance of species and the enhancement of freezing resistance of cut-tings, and may play an important role in the antifreeze process under freezing temperature, which would provide the basis for thestudy on the molecular mechanism of freezing resistance in P. suaveolens and the cloning of gene associated with freezing resistance.关键词
P. suaveolens, P. tomentosa, freezing acclimation, freezing resistance, LT50, G6PDHKey words
P. suaveolens, P. tomentosa, freezing acclimation, freezing resistance, LT50, G6PDH分类
农业科技引用本文复制引用
Lin Shanzhi,Zhang Zhiyi,Lin Yuanzhen..Comparison of G6PDH Activity and LT50 Between P. tomentosa and P.suaveolens During Freezing Acclimation[J].中国林学(英文版),2003,5(1):20-24,5.基金项目
Supported by the National Natural Science Foundation of China (Grant No. 30271093) （Grant No. 30271093）
