中国实验动物学报2001，Vol.9Issue(1)：20-25,6.
嗜肺巴斯德杆菌选择性培养基研制及应用
The Formulation and Application of a Selective Medi um for Identification of Pasteurella pneumotropica
摘要
Abstract
Objective To develop a selective m edium (PPSM) that shows strongselection to P.pneumotropica in conventional diagnosis.Methods Drug sensitivity test and MIC were used.Results A selective medium(PPSM) and its selective b roth(PP broth) for Pasteurella pneumotropica were devel oped.After incubation o n PPSM at 37℃ for 48 hr，P.pneumotropica formed speci al colony about 1mm in dia meter which was convex，damp，grey or black with the luster of metals.Its inhi bitory rate to Staphylococcus epidermidis and E .coli was 100%，to Proteus sp wa s 76%，and the characteristic swarming growth was inhibited.After incubation in PP broth，PPSM made the positive rate of feces from SPF mice increased from 0 to 67.2%.Primary culture was almost pure when mice laryngopharyngeal swabs inoculated on PPSM.Conclusion The medium was strongly selective to P.pneumotropica.It could be very easy and correct for the diagnosis of P.pneumotropica without sacrificing animals.关键词
嗜肺巴斯德杆菌/选择性培养基分类
生物科学引用本文复制引用
李红,刘星,张丽芳,许虎峰..嗜肺巴斯德杆菌选择性培养基研制及应用[J].中国实验动物学报,2001,9(1):20-25,6.基金项目
本课题受北京市科委实验动物科学专项基金资助(953991200)。 （953991200）
