生态学报2005，Vol.25Issue(2)：203-209,7.
两个不同生态特征蝗区东亚飞蝗的两种代谢酶
Two metabolic enzymes of oriental migratory locust, Locusta migratoria mani-lensis (Meyen), from two locust areas with different ecological characteristics
摘要
Abstract
Huanghua and Pingshan are two different locust swarming areas in Hebei, China. Huanghua is sea-coast locust area and Pingshan is lake-shored area. Huanghua is a plain in Hebei province close to Bohai bay, where Locusta migratoria manilensis (Meyen) feeds on Phragmites communis (Trin). Frequent massive outbreaks of Locusta migratoria manilensis (Meyen) in Huanghua make it an important area of locust pest control in China. Pingshan is a mountainous area, and collection place is verge of Gangnan reservoir. Locusta migratoria manilensis (Meyen) feed on Echinochloa crusgaii (L.) Beauv and other crops like corn and legume in Pingshan, and chemicals have been rarely used for locust control for water source protection. General esterases and glutathione S-transferase (GST) of oriental migratory locust, Locusta migratoria manilensis (Meyen), collected from the two localities were compared. Inhibition studies of the esterases using four inhibitors (i. e., paraoxon, malaoxon, eserine, and carbaryl) indicated that most of general esterases in the two populations were B-type. Using α-NA, α-NB and β-NA as substrates, the activities of esterases were found 1.63-, 1.66-, and 1.70-fold higher for females from Huanghua population and 1.12-, 1.41-, and 1.27-fold higher for males from Huanghua population than those from Pingshan population. Meanwhile Huanghua population had more individuals with high esterase activity than Pingshan population. The difference of LD50 value for malathion between the two populations was consistent with the difference of esterase activities, suggesting that general esterases contributed to the malathion resistance of the two populations. The spectrum of esterase activities suggested that Huanghua population had higher potential resistance level than Pingshan population. However, the GST activity of Huanghua population was lower than that of Pingshan population. We suggested that GST activity was not responsible for the malathion resistance of Locusta migratoria manilensis (Meyen).关键词
蝗区/酯酶/谷胱甘肽S-转移酶/杀虫剂抗性/东亚飞蝗Key words
locust area/esterase/GST/insecticide resistance/oriental migratory locust/Locusta migratoria manilensis (Meyen)分类
生物科学引用本文复制引用
贺艳萍,刘新,马恩波..两个不同生态特征蝗区东亚飞蝗的两种代谢酶[J].生态学报,2005,25(2):203-209,7.基金项目
国家自然科学基金资助项目(30170612) （30170612）
国家自然基金国际合作资助项目(30210103147) （30210103147）
山西省科技攻关资助项目(012015) （012015）
