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携有抗白叶枯病新基因Xa23水稻近等基因系的构建及应用

章琦 王春连 赵开军 杨文才 乔枫 周永力 江祺祥 刘古春

中国水稻科学2002,Vol.16Issue(3):206-210,5.
中国水稻科学2002,Vol.16Issue(3):206-210,5.

携有抗白叶枯病新基因Xa23水稻近等基因系的构建及应用

Development of Near-Isogenic Line CBB23 with a New Resistance Gene to Bacterial Blight in Rice and Its Application

章琦 1王春连 1赵开军 1杨文才 1乔枫 1周永力 1江祺祥 2刘古春2

作者信息

  • 1. 中国农业科学院,作物育种栽培研究所,农业部作物遗传育种重点实验室,北京,100081
  • 2. 江苏省武进市稻麦育种场,江苏,武进,213149
  • 折叠

摘要

Abstract

The near-isogenic line (NIL) with a new gene Xa23 for resistance to bacterial blight (BB) was developed and designated as CBB23 in 1993-1998. A susceptible indica variety JG30 with improved plant type was used as a recurrent parent. The progenies derived from the cross JG30/H4 harboring the gene Xa23 were developed by back-cross until BC5, and selfing for 4 times. Evaluation of the resistance gene Xa23 with specific strain P6 and selection of plant type successively were carried out till the line showing stable resistance and agronomic characters similar to the recurrent parent. Comparison of reaction for resistance spectrum to 20 strains including 10 Philippine races, 3 Japanese races, and 7 Chinese pathotypes between CBB23(carrying Xa23 )and IRBB21(carrying Xa21) was made. Xa23 is resistant to all 20 strains, and Xa21 is resistant to 19 strains and highly susceptible to the Philippine race 10. The NIL CBB23 was used to establish segregation populations of JG30/CBB23 for mapping the gene Xa23. The gene was located on rice chromosome 11 in the preliminary tagging by screening SSR. Three AFLP markers screened are closely linked with Xa23. The genetic distance between APKj23 and Xa23 is about 1.0 cM. The NIL is also valuable in rice breeding.

关键词

近等基因系/育种/抗性/基因/白叶枯病/水稻

Key words

near-isogenic line/breeding/resistance/gene/bacterial blight/rice

分类

农业科技

引用本文复制引用

章琦,王春连,赵开军,杨文才,乔枫,周永力,江祺祥,刘古春..携有抗白叶枯病新基因Xa23水稻近等基因系的构建及应用[J].中国水稻科学,2002,16(3):206-210,5.

基金项目

Supported by the Rockefeller Foundation(洛克菲勒基金资助项目)(94001#253,97001#258) (洛克菲勒基金资助项目)

the National Natural Science Foundation of China(国家自然科学基金资助项目)(39670508) and a grant from the Ministry of Agriculture(农业部科技项目)(J00-A-002). (国家自然科学基金资助项目)

中国水稻科学

OA北大核心CSCDCSTPCD

1001-7216

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