中国现代医学杂志2006，Vol.16Issue(14)：2081-2086,6.
通腑法通腑利肺作用机制的实验研究
Study on mechanism of protective effect on lung by method of Tongfu
摘要
Abstract
[Objective] To explore the mechanism of protective effect on the lung by the method of Tongfu through the three levels of general, cell and molecular. [Methods] Forty SD rats were randomly divided into four groups: normal control group, model group, ligation group and treatment group (n=10 each). The models were made by the method of incomplete ligating the rectum outside the body. The content of IL-8 in blood serum of each group was measured by the method of radioactive immunity. The expression of TNF-αmRNA in lung tissues was detected by RT-PCR method, and the percentage of the dead pulmonary alveolar macrophage (PAM) in the bronchoalveolar lavage fluid (BALF) was detected. In each group, the results were compared among the four groups. [Results] The content of IL-8 in blood serum and the expression of TNF-α mRNA in lung tissues in the model rats were significantly higher than those in the normal control rats (P ＜0.01). After the treatment of Tongfu, all of the indexes decreased and the results were lower than those in the model rats, and damage of the lung in the treatment group was reduced. [Conclusion] IL-8 and TNF-α play an important role during the process of intestinal endotoxemia caused lung damage, the method of Tongfu will protect the lung from damage.关键词
Il-8/TNF-α/肺泡巨噬细胞/通腑Key words
IL-8/TNF-α/pulmonary alveolar macrophage/Tongfu分类
医药卫生引用本文复制引用
杨胜兰..通腑法通腑利肺作用机制的实验研究[J].中国现代医学杂志,2006,16(14):2081-2086,6.基金项目
This study was supported by Hubei Department of Health(NO.3-98) （NO.3-98）
