中国现代医学杂志2005，Vol.15Issue(14)：2084-2087,4.
结核分枝杆菌含信号肽的Mtb8.4基因的克隆及真核表达质粒的构建
Cloning of Mtb8.4 with signal peptide gene and construction of pcDNA3.1(+)-MS eukaryotic expression plasmid
李晖 1邹永胜 1钟森 1任红2
作者信息
- 1. 泸州医学院附属医院,感染病科,四川,泸州,646000
- 2. 重庆医科大学第二临床学院,重庆,400010
- 折叠
摘要
Abstract
[Objectives] To construct and identify the pcDNA3.1(+)-MS eukaryotic expression plasmid. [Methods] Extracted DNA from M. Tuberculosis was amplified by PCR and the target gene we got was cloned into the unique HindⅢ and EcoR Ⅰ cloning sites of pcDNA3.1(+). [Results] The accuracy of pcDNA3.1(+)-MS plasmid constructs was confirmed by a series of molecular biology techniques. [Conclusion] The construction of pcDNA3.1(+)MS provided the possibility for investigating immunogenicity of the recombinant plasmid, studying on the role of the signal peptide in the protein expression and excretion and preparing a new tuberculosis vaccine.关键词
结核杆菌/MS/PCR/基因克隆Key words
M. Tuberculosis/MS/PCR/clone分类
医药卫生引用本文复制引用
李晖,邹永胜,钟森,任红..结核分枝杆菌含信号肽的Mtb8.4基因的克隆及真核表达质粒的构建[J].中国现代医学杂志,2005,15(14):2084-2087,4.
