大豆科学2000，Vol.19Issue(3)：210-217,8.
大豆DNA扩增片段长度多态性(AFLP)研究
A STUDY ON AMPLIFIED FRAGMENT LENGTH POLYMORPHISM (AFLP) IN SOYBEAN
摘要
Abstract
In the present paper, improvement of the DNA extraction from dry soybean seed, comparison between the silver staining and isotope labeling methods, and exploration of the endoenzyme and primer combinations suitable for AFLP analysis in soybeans were presented. Based on this, a simple and fast procedure suitable for soybean AFLP analysis was established,and the representative accessions of G. soja and G. max from various ecologi- cal areas in China were examined by using the suggested procedure.关键词
大豆/DNA提取/扩增片段长度多态性(AFLP)/银染检测Key words
Soybean/DNA extraction/Silver－staining inspection/AFLP (amplified frag- ment length polymorphism)分类
农业科技引用本文复制引用
田清震,盖钧镒,喻德跃,贾继增..大豆DNA扩增片段长度多态性(AFLP)研究[J].大豆科学,2000,19(3):210-217,8.基金项目
国家"九五"重点科技项目(No．9601401064) National Key Science and Technology Project No. 960140106 （No．9601401064）
