中国动脉硬化杂志2001，Vol.9Issue(1)：21-23,3.
神经肽Y诱导血管平滑肌细胞凋亡相关基因表达与细胞内液pH变化的关系
The Relationship between Expression of Apoptosis-Related Genes Induced by Neuropeptide Y and Changes of Intracellular pH in Vascular Smooth Muscle Cells
摘要
Abstract
Aim To explore the role of changes of intracellular pH inexpression of apoptosis-related genes of cultured vascular smooth cells induced by neuropeptide Y. Methods The rat vascular smooth muscle cells were used for experiments at passage 4 to 6. The expression of bcl-2 and bax were quantitatively detected in cultured vascular smooth muscle cells and the intracellular pH were also quantitatively detected with the immunofluorescent quantitative skill through Laser scanning confocal microscope (ACAS570). Results It was found that at the action of NPY (10-6mmol/L), bcl-2 and bax were markedly expressed in cultured smooth muscle cells compared with control group. The fluorescent value of bcl-2 and bax were 1 349.67±108.28 and 1397.50±104.43, much higher than 921.72±80.31 and 1 060.44±107.54 in the control group respectively (P<0.01). Intracellular fluid pH in VSMC was decreased by NPY. Conclusion The expression of apoptosis-related genes induced by neuropeptide Y is mediated, in part, by intracellular acidosis in cultured vascular smooth muscle cells.关键词
神经肽Y/细胞内液/酸化/肌,平滑,血管/凋亡/大鼠分类
医药卫生引用本文复制引用
刘建康,胡必利,梁若斯,陈敏生,黄少华..神经肽Y诱导血管平滑肌细胞凋亡相关基因表达与细胞内液pH变化的关系[J].中国动脉硬化杂志,2001,9(1):21-23,3.基金项目
广东省自然科学基金(980466)和广州市科委基础研究项目(99-J-009-01)资助 （980466）
