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Hydrogen Production with High Evolution Rate and High Yield by Immobilized Cells of Hydrogen-producing Bacteria Strain B49 in a Column Reactor

高技术通讯(英文版)2002,Vol.8Issue(4):21-25,5.
高技术通讯(英文版)2002,Vol.8Issue(4):21-25,5.

Hydrogen Production with High Evolution Rate and High Yield by Immobilized Cells of Hydrogen-producing Bacteria Strain B49 in a Column Reactor

Hydrogen Production with High Evolution Rate and High Yield by Immobilized Cells of Hydrogen-producing Bacteria Strain B49 in a Column Reactor

1

作者信息

  • 1. School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, P.R China;Research Center of Life Science and Biotechnology, Northeast Agricultural University, Harbin 150030, P.R China;School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, P.R China;School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, P.R China;School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, P.R China;School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, P.R China
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摘要

Abstract

To improve the hydrogen evolution rate in continuous hydrogen production of a novel fermentative hydrogen-producing bacteria strain B49 (AF481148 in EMBL), 4 % immobilized cells by polyvinyl alcohol-boric acid method, with the addition of a small amount of calcium alginate in a column reactor obtain hydrogen yield of 2.31 mol H2/mol glucose and hydrogen evolution rate of 1435.4 ml/L culture*h respectively at medium retention time of 2.0 h with a medium containing 10g glucose/L. Moreover, as the cell density in gel beads is increased to 8%, hydrogen yield and hydrogen evolution rate for 10g glucose/L are 2.34 mol H2/mol glucose and 2912.4 ml/L culture*h respectively at medium retention time of 1.0 h, and for molasses wastewater COD of 7505.9 mg/L hydrogen production potential of 205.6 ml/g COD and hydrogen evolution rate of 2057.7 ml/L culture*h at hydraulic retention time of 0.75 h are observed. In the continuous culture pH value keeps around 3.9 by self-regulating.

关键词

hydrogen-producing bacteria strain B49,immobilized cells,continuous hydrogen production

Key words

hydrogen-producing bacteria strain B49,immobilized cells,continuous hydrogen production

分类

能源科技

引用本文复制引用

..Hydrogen Production with High Evolution Rate and High Yield by Immobilized Cells of Hydrogen-producing Bacteria Strain B49 in a Column Reactor[J].高技术通讯(英文版),2002,8(4):21-25,5.

基金项目

Sponsored by "863"Project of China (2001AA515030) &"973" Fundamental Science Program of China (G2000026402) (2001AA515030)

高技术通讯(英文版)

OAEI

1006-6748

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