陕西科技大学学报（自然科学版）2008，Vol.26Issue(4)：21-26,6.
一种检测濑尿虾中镉残留的直接ELISA
A DIRECT ELISA TO QUANTIFY CADMIUM RESIDUE IN RED-BANDED MANTIS SHRIMP
摘要
Abstract
In order to establish a fast and simple assay of detecting cadmium residue, a direct competitive enzyme-linked immunosorbent assay (DC-ELISA) based on a cadmium-specific monoclonal antibody and enzyme labeled antigen was developed. The DC-ELISA was capable of detecting cadmium ion as low as 0.20 μg/L and the IC50 of DC-ELISA was 3.01 μg/L. The cross reactivity exhibited by the tested metal-chelates was < 1.6 %. The overall coefficient of variations of DC-ELISA was 6.67 %~9.09 %. The coefficient correlation of results between DC-ELISA and GFAAS was 0.988. The results indicated DC-ELISA could detect cadmium residue in red-banded mantis shrimp with high sensitivity and accuracy.关键词
镉/抗原/单克隆抗体/酶联免疫吸附测定/濑尿虾Key words
cadmium/antigen/monoclonal antibody/ELISA/red-banded mantis shrimp分类
轻工业引用本文复制引用
刘功良,王菊芳,李志勇,梁世中..一种检测濑尿虾中镉残留的直接ELISA[J].陕西科技大学学报（自然科学版）,2008,26(4):21-26,6.基金项目
广东省科技计划项目(2003C20409),国家质检总局科技项目(2004IK062) （2003C20409）
