同位素2001，Vol.14Issue(1)：21-26,6.
153Sm标记平阳霉素偶联物
Conjugated Bleomycin A5-DTPA and Its Labeling With 153Sm
蒋树斌 1罗顺忠 1谯健 1魏洪源 1何佳恒1
作者信息
摘要
Abstract
Bleomycin A5(BLMA5) is conjugated with cyclic DTPA anhydride andlabeled with 153Sm. The conditions of conjugation and labeling are investigated. The possible composition of BLMA5-DTPA is analyzed by measurement and computation. The results show that bleomycin A5 can conjugate with DTPA anhydride easily when the mole ratio of cDTPAA to BLMA5 is more than 10 in weak basic media, and the conjugating yield is more than 80%;A molecule of BLMA5 conjugate with 2 molecule of cDTPAA when the mole ratio of cDTPAA to BLMA5 is not less than 15, and the conjugative positions is on 2 different N atoms. The results of labeling experiments indicate that the labeling yield of 153Sm-DTPAA-BLMA5 is 98% at pH7～9 and room temperature for 30 min when the mole ratio of DTPA-BLMA5 to 153Sm is more than 8. 153Sm-BLMA5-DTPA is stable and the radiochemical purity is more than 96% at room temperature for 6 days.关键词
153Sm/平阳霉素/cDTPAA/偶联/标记分类
化学化工引用本文复制引用
蒋树斌,罗顺忠,谯健,魏洪源,何佳恒..153Sm标记平阳霉素偶联物[J].同位素,2001,14(1):21-26,6.
