水生生物学报2001，Vol.25Issue(1)：21-27,7.
草鱼、中华鳖脾细胞培养上清液IL-2物质的检测
IDENTIFICATION OF AN INTERLEUKIN-2 SUBSTANCE
IN SPLENOCYTE CULTURE SUPERNATANT OF GRASS CARP
CTENOPHARYNGODON IDELLUS AND CHINESE SOFT-SHELLED
TURTLE TRIONYX SINENSIS
摘要
Abstract
Interleukin-2(IL-2) activity by thymocyte (C57BL/6-8 mice) proliferation assay and fibroblast(L929 fibroblasts) cytotoxicity assay has been found in supernatant of mitogen(ConA)-stimulated splenocyte cultures of grass carp Ctenophaxyngodon idellus, Chinese soft-shelled turtle Trionyx sinensis.This IL-2 activity was neutralized by polyclonal anti-human IL-2 antisera.Besides, the splenocyte culture supernatant(IL-2) of Trionyx sinensis increased obviously thymocyte(Trionyx sinensis) proliferation,which was also neutralized by polyclonal anti-human IL-2 antisera,and eliminated the suppressive effect of rabbit anti-thymocyte(Trionyx sinensis) antisera (RATTS) on thymocyte (Trionyx sinensis).Further,with indirect immunohistochemistry technique,CD25(IL-2 receptor or IL-2R) cross-reaction has been identified on lymphocyte membrane of Ctenophaxyngodon idellus and Trionyx sinensis using mouse monoclonal antibody directed against Human Leucocyte Differentiation Antigen CD25(IL-2R).关键词
白细胞介素-2/白细胞介素-2受体/发育和比较免疫学/草鱼/中华鳖分类
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郭琼林..草鱼、中华鳖脾细胞培养上清液IL-2物质的检测[J].水生生物学报,2001,25(1):21-27,7.
