中国临床康复2005，Vol.9Issue(4)：213-215,3.
艾滋病相关痴呆机制研究:鼠海马脑片 CA1区突触传递及可塑性与 HIV-1包膜糖蛋白 gp120的关系
Mechanism study of acquired immunodeficiency syndrome associated dementia:the relationship between the synaptic transmission and plasticity of hippocampal CA1 area in rats and human immunodeficiency virus 1 enveloped protein gp120
摘要
Abstract
BACKGROUND:No particularly effective medicine could treat human immunodeficiency virus 1(HIV 1) associated dementia(HAD) at present,which mainly because the mechanism of HIV 1 infection induced neural damage and necrosis is still not completely clarified. OBJECTIVE:To investigate the effects of human immunodeficiency virus I type(HIV 1) enveloped protein(EP) gp120 on the synaptic transmission and plasticity in hippocampal CA1 area of rat to clarify the mechanism of HAD generation. DESIGN:A paired design. SETTING:Department of Pathophysiology of Medical College of Jinan University. MATERIALS:The study was conducted in the Department of Pathophysiology(a tertiary national key laboratory of National Administration for Traditional Chinese Medicine,registration number: TCM 03 131) of the Medical College of Jinan University from January to October of 2003.Male SD rats aged between 2 and 5 weeks were used in the study. INTERVENTIONS:Brain slice perfusion and recording technique was employed. MAIN OUTCOME MEASURES:To record the excitatory postsynaptic potential(EPSP) in hippocampal CA1 area in rat;to investigate the effects of gp120 on long term potentiation(LTP) induced by high frequency electric stimulation in Schaffer lateral branch. RESULTS:gp120 had inhibitive effect on LTP in hippocampal CA1 area in rat [the average amplitude of LTP decreased from normal (216.1± 14.0) % to(90.8± 6.0) % ,n=12,P< 0.01],but had no effect on basic EPSP.PKA/PKC protein kinase(PK) inhibitor H7 could reverse this inhibitive effect [the average amplitude of LTP was(198.8± 16.2) % ,n=8,P< 0.01]. CONCLUSION:gp120 might participate the generation of HAD through inhibiting LTP in hippocampal CA1 area.关键词
HIV1/HIV1包膜糖蛋白Gp120/长时程增强/海马分类
医药卫生引用本文复制引用
董军,潘锐,沈伟哉..艾滋病相关痴呆机制研究:鼠海马脑片 CA1区突触传递及可塑性与 HIV-1包膜糖蛋白 gp120的关系[J].中国临床康复,2005,9(4):213-215,3.基金项目
广东省医学科研基金项目 (A2004327) （A2004327）
广东省自然科学基金项目 (04010443) （04010443）
广东省自然科学基金团队项目 (039213) （039213）
