中德临床肿瘤学杂志(英文版)2008，Vol.7Issue(4)：213-216,4.
苦参碱对HepG2细胞杀伤作用及机制研究
Effect of matrine on HepG2 cells:role of glutathione and cytochrome c
摘要
Abstract
Objective:To investigate the death mode of human hepatoma cells exposed to matrine and the role of glutathione (GSH) and cytochrome c.Methods:The MTT test and Cell Death Detection ELISA were used to identify cell death mode and viability of cells exposed to matrine.The volume of intracellular GSH was detected by GSH reductase.Finally Western blotting was chosen to analyze the expression of cytochrome c and Caspase-9 in HepG2 cells treated by matrine.Results:The apoptotic cell death induced by matrine in Hep G2 cells dramatically increased in the time-,dose-dependent manner.Matrine can exhaust intracellular GSH effectively to change the redox state in cells.Furthermore it affect the cytotoxicity of matrine.Results of Western blotting showed that matrine induced the release of cytochrome c from mitochondria to cytoplasm,and then stimulate the cleavage of Cespese-9 in a time-dependent manner.Conclusion:Matrine induced apoptosis in Hep G2 cells through the mitochondrial pathway,and oxidative stress via depletion of GSH is directly involved in the apoptotic process.关键词
matrine/glutathione/cytochrome c/Caspase-9/apoptosisKey words
matrine/glutathione/cytochrome c/Caspase-9/apoptosis分类
医药卫生引用本文复制引用
Xiangdong Cheng,Yian Du,Ling Huang,Zhiming Jing,Zhiguo Zheng..苦参碱对HepG2细胞杀伤作用及机制研究[J].中德临床肿瘤学杂志(英文版),2008,7(4):213-216,4.基金项目
Supported by a grant from the Chinese Traditional Medicine Foundation of Zhejiang (No.2007CB184). （No.2007CB184）
