中华医学杂志（英文版）2008，Vol.121Issue(21)：2134-2138,5.
Effects and mechanism of glucagon-like peptide-1 on injury of rats cardiomyocytes induced by hypoxia-reoxygenation
Effects and mechanism of glucagon-like peptide-1 on injury of rats cardiomyocytes induced by hypoxia-reoxygenation
XIE Yun 1WANG Shao-xin 1SHA Wei-wei 1ZHOU Xue 1WANG Wei-lin 2HAN Li-pin 2LI Dai-qing 3YU De-min3
作者信息
- 1. Department of Diabetic Neurology,Metabolic Disease Hospital,Tianjin Medical University,Tianjin 300070,China
- 2. Department of Endocrinology,Beijing Changping DistrictHospital,Beijing 102200,China
- 3. Department of Endocrinology,Chinese People's Liberation Army252 Hospital,Baoding,Hebei 071000,China
- 折叠
摘要
Abstract
Background Although the insulinotropic role of glucagon-like peptide-1 (GLP-1) in type 2 diabetes mellitus has been substantiated,its role in cardioprotection remains largely unknown.This study aimed to determine the effects of GLP-1 on injury of rats cardiac myocytes induced by hypoxia-reoxygenation (H/R) and the possible mechanisms.Methods The cultured neonatal rats cardiac myocytes were randomly divided into seven groups:the normal control group,the H/R group,the GLP-1+H/R group,the GLP-1+H/R+UO126 (the p42/44 mitogen-activated protein kinase (MAPK) inhibitor) group,the GLP-1+H/R+LY294002 (phosphatidylinositol 3-kinase (P13K) inhibitor) group,the H/R+UO126 group,and the H/R+LY294002 group.The lactate dehydrogenase (LDH) activity,apoptosis rate of cardiac myocytes,and caspase-3 activity were detected after the injury of H/R.Results Compared with the normal control group,the activity of LDH,cardiac myocyte apoptosis rate,and caspase-3 activity all increased significantly in the H/R group (P<0.01).Compared with the H/R group,these three indices all decreased in the H/R+GLP-1 group (P<0.01).However,the changes of LDH activity,apoptosis rate,and caspase-3 activity were inhibited by LY294002 and UO126 respectively.Conclusions GLP-1 can directly act on cardiac myocytes and protect them from H/R injury mainly by inhibiting their apoptosis.Its mechanism may be through the P13K-Akt pathway and the MAPK signaling pathway.关键词
glucagon-like peptide-1/hypoxia-reoxygenation/apoptosis/caspase-3/MAP kinase/P13K/AktKey words
glucagon-like peptide-1/hypoxia-reoxygenation/apoptosis/caspase-3/MAP kinase/P13K/Akt分类
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XIE Yun,WANG Shao-xin,SHA Wei-wei,ZHOU Xue,WANG Wei-lin,HAN Li-pin,LI Dai-qing,YU De-min..Effects and mechanism of glucagon-like peptide-1 on injury of rats cardiomyocytes induced by hypoxia-reoxygenation[J].中华医学杂志（英文版）,2008,121(21):2134-2138,5.
