首页|期刊导航|中国海洋湖沼学报（英文版）|Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri
中国海洋湖沼学报（英文版）2008，Vol.26Issue(2)：215-218,4.
Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri
Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri
摘要
Abstract
Two Large-insert genomic bacterial artificial chromosome (BAC) libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research.High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I,respectively.The BamH I library consisted of 53760 clones while the Mbo I library consisted of 7680 clones.Approximately 96% of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb,providing a coverage of 5.3 haploid genome equivalents.Similarly,the Mbo I library with an average insert of 145 kb and no insert-empty clones,thus providing a genome coverage of 1.1 haploid genome equivalents.关键词
BAC library/Zhikong scallop/Chlamys farreri/genomeKey words
BAC library/Zhikong scallop/Chlamys farreri/genome分类
海洋科学引用本文复制引用
ZHANG Yang,ZHANG Xiaojun,Chantel F.SCHEURING,ZHANG Hongbin,LI Fuhua,XIANG Jianhai..Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri[J].中国海洋湖沼学报（英文版）,2008,26(2):215-218,4.基金项目
Supported by Key Program of National Natural Science Foundation of China (No.30730071) （No.30730071）
Major State Basic Research Development Program of China (2006CB101804) （2006CB101804）
