中国科学院研究生院学报2007，Vol.24Issue(2)：217-222,6.
STZ诱导的糖尿病对骨密度和血清睾酮的影响
Effect of STZ-induced diabetes on bone mineral density and testosterone
摘要
Abstract
To investigate the effect of diabetes mellitus on bone metabolism,bone mineral density(BMD)and serum testosterone were studied in STZ-induced diabetic male rats. Male Wistar rats were randomly divided into control group ( n = 6), diabetic group ( n = 5 ) and insulin-treated diabetic group ( n = 5 ).Diabetes was intravenously induced by injection of streptozotocin(STZ, 50mg/kg body weight), confirmed by 12h fasting blood glucose concentration( 12mmol/L). The treatment group was subcutaneously injected with protamine zinc insulin( 1.8 ～ 2.2U/animal)at the same time every day. The experiment lasted 32d.Femoral BMD was determined by dual-energy X-ray absorptiometry (DEXA). Serum testosterone was detected by Enzyme-linked Immunosorbent Assay(ELISA)kit. Total alkaline phosphatase(ALP), calcium (Ca) and phosphorus (P)in serum were measured by bio-machine. Femoral BMD in diabetic group was significantly lower than that in control( P ＜ 0.01 ) but reserved by insulin treatment. Testosterone in serum was also reduced in diabetic rats( P ＜ 0.01 ) and normalized by insulin treatment. The serum ALP concentration was markedly higher in diabetics( P ＜ 0.01 ) compared with the controls and prevented by insulin treatment. However, no significant differences were observed in serum Ca and P among three groups. Our results suggest that BMD is seriously affected by diabetes. Insulin deficiency and testosterone decrease are important causes of diabetic osteoporosis. And insulin treatment prevented bone loss and testosterone depression.关键词
骨质疏松/糖尿病/骨密度/睾酮Key words
osteoporosis/diabetes mellitus/bone mineral density/testosterone分类
医药卫生引用本文复制引用
费聿荣,韦丹,李明,张敏,张晓琳,丁文军,杨建虹..STZ诱导的糖尿病对骨密度和血清睾酮的影响[J].中国科学院研究生院学报,2007,24(2):217-222,6.基金项目
supported by the Scientific Research Foundation of Graduate University of Chinese Academy of Sciences(055101FM03)and China National Natural Sciences Foundation(20571084) （055101FM03）
