郧阳医学院学报2009，Vol.28Issue(3)：218-221,4.
过表达小窝蛋白-1对293T细胞生长的影响及机制
Effect of Caveolin-1 Overexpression on the Growth of 293T Cells and Its Mechanism
杞少华 1李东升 1李瑞明 1武栋成2
作者信息
- 1. 郧阳医学院附属太和医院湖北省胚胎干细胞研究重点实验室,湖北十堰,442000
- 2. 武汉大学医学院,湖北武汉,430071
- 折叠
摘要
Abstract
Objective To investigate the effects of caveolin-1 overexpression on the growth and proliferation of 293T cells and its mechanism.Methods Recombinant plasmid PHLCX Nflag3/caveolin-1 was transfected into 293T cell by liposome method,the cell cycle Was detected by FCM;the expression of P53,P21,ERK and pp-ERK was detected with Western Blotting analysis.Results The overexpression of caveolin-1 arrested 293T cells in GO/G1 phase of the cell cycle;the expression of P53,P21 increased and the expression of ERK and pp-ERK decreaSed.Conclusion Overexpression of caveolin-1 may inhibit the growth of 293T cells,P53/P21 and ERK signal transduction pathway may involve in the inhibitory effect.关键词
小窝蛋白-1/基因表达/293T细胞/P53/P21/ERK/ppERKKey words
Caveolin-1/Gene expression/293T cell/P53/P21/ERK/ppERK分类
生物科学引用本文复制引用
杞少华,李东升,李瑞明,武栋成..过表达小窝蛋白-1对293T细胞生长的影响及机制[J].郧阳医学院学报,2009,28(3):218-221,4.
