国际眼科杂志2008，Vol.8Issue(2)：219-222,4.
Transwell小室共培养条件下RPE促进 Müller细胞的增殖和迁移
Promotion of proliferation and migration of Müller cells by RPE cells in a co-culture system
马洪梅 1张晓梅 1付小玻 1李伟军 1乌兰 1王巍1
作者信息
- 1. 150001,中国黑龙江省哈尔滨市,哈尔滨医科大学第一临床医学院附属眼科医院
- 折叠
摘要
Abstract
AIM: To investigate the role of retinal pigment epithelium (RPE) in the growth of Müller cells using a co-culture system in vitro . METHODS: Müller cells were cocultured with RPE cells under both normoxic and hypoxic conditions in Transwell chamber culture system. Müller cell proliferation was evaluated by MTT assay. The number of cells which migrate through micropores and stay on the outer bottom side of insert systems were observed and counted. RESULTS: The activities of proliferation and migration of Müller cells when cocultured with RPE cells were significantly higher than those of the Müller cells when cultured alone at all time points under both normoxic and hypoxic conditions. However, for both the coculture and control groups, there is no significant difference between the measurements at 3 and 6 hours. CONCLUSION: Evidence suggests that RPE, when co-cultured with Müller cells, can stimulate migration and proliferation of Müller cells under both hypoxic and normoxic conditions in a time-dependent manner; how-ever, there is no evidence to support the synergetic interaction of RPE and Müller cells co-cultured under hypoxic conditions.关键词
Transwell小室/缺氧/视网膜色素上皮细胞/Müller细胞Key words
Müller cells/ retinal pigment epithelium/ co-culture/ hypoxia/ normoxia/ Transwell system分类
医药卫生引用本文复制引用
马洪梅,张晓梅,付小玻,李伟军,乌兰,王巍..Transwell小室共培养条件下RPE促进 Müller细胞的增殖和迁移[J].国际眼科杂志,2008,8(2):219-222,4.
