中德临床肿瘤学杂志（英文版）2009，Vol.8Issue(4)：219-224,6.DOI:10.1007/s10030-009-0011-0
放疗敏感性不同宫颈癌的蛋白质组学比较研究
Comparative proteomic study of cervical cancer with different radiotherapy sensitivity
摘要
Abstract
Objective: To investigate the proteomic differences between the high-sensitivity (HS) group and low-sensitivity (LS) group of cervical cancer treated by radiotherapy and confirm the radiotherapy sensitivity associated proteins in early cer-vical cancer. Methods: The fresh carcinoma tissues were collected from 10 untreated cervical cancer patients and preserved in the-80℃ refrigeratory. The tissues were classified into two groups: high sensitivity group (HS) and low sensitivity group (LS), according to their response to radiotherapy. In the first part of our experiment, protein separating was performed by using two-dimensional gel electrophoresis (2-DE) with Amersham 18 cm linear pH 3-10 immobilized pH gradient (IPG) strips. The images of the gels were acquired by the scanner and then analyzed by using PD-quest7.3 software to find the differentially expression protein-spots in each group. Then the differentially expressed protein-spots was incised from the gels and digested by trypsin. The peptide mass fingerprintings (PMF) was acquired by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and the proteins were identified by data searching in the Mascot-database. Part of dif-ferentially expression proteins were assayed by Western Blot. Results: Most of the gels were clear and successfully analyzed by PD-quest7.3 software. Most of the protein-spots concentrated on the area of 20-100Kda (Mw) and pH4-8. The averagetwo groups. Five high expression proteins were found in HS group which were low expression in LS group, 3 high expres-z sion protein were found in LS group which were low expression in HS group. Reselts of Western Blot were in coincidence to proteomic result. Conclusion: The 2-DE gels image of HS group and LS group with early cervical cancer tissues treated by radiotherapy are successfully acquired. Some differentially expression proteins between the two groups are further confirmed by immunohistochemical assay.关键词
cervical cancer/radiotherapy, proteomics/two-dimensional gel electrophoresis/mass spectrometryKey words
cervical cancer/radiotherapy, proteomics/two-dimensional gel electrophoresis/mass spectrometry分类
医药卫生引用本文复制引用
Liang Zeng,Hong Zhu,Dajun Li,Haiping Pei,Yaping Deng,Jun Yuan..放疗敏感性不同宫颈癌的蛋白质组学比较研究[J].中德临床肿瘤学杂志（英文版）,2009,8(4):219-224,6.基金项目
Supported by grants from the Hunan Natural Science foundation(No. 06JJ4199) and the Hunan Science Technology Foundation (No.2007SK3010). （No. 06JJ4199）
