中国临床康复2005，Vol.9Issue(5)：220-221,2.
全脑缺血再灌注损伤大鼠早期脑皮质超微结构的变化
Ultrastructural changes of brain cortex in rats at early stage of global ischemia reperfusion injury
王涛 1阚清 2罗芳 2赵继宗 1孙异临3
作者信息
- 1. 首都医科大学附属北京天坛医院,神经外科,北京市,100050
- 2. 首都医科大学附属北京天坛医院,麻醉科,北京市,100050
- 3. 首都医科大学附属北京天坛医院,神经外科研究所电镜室,北京市,100050
- 折叠
摘要
Abstract
BACKGROUND: Quiet a number of researches has reported the morphological changes of global ischemic reperfusion model. However, there are few reports on the ultrastructural changes of cortex in early reperfusion, especially the change of blood brain barrier.OBJECTIVE: To explore the changes of brain cortex neurons, glial cells and blood brain barrier in order to provide reliable evidence for clinical treatment.DESIGN: A randomized and controlled trial.SETTING: Department of Neurosurgery, Departnent of Anesthesia and Electron Microscope Room of Beijing Tiantan Hospital.MATERIALS: The experiment was conducted to 6 Wistar rats in Beijing Neurological Surgery Research Institute of Capital University of Medical Sciences during February 2003 to February 2004. The rats were randomly divided into two groups with one of ischemia-reperfusion group and sham operation group with 3 rats in each group.INTERVENTIONS: To prepare global ischemic reperfusion model of rats. Brain was removed from ischemic group in one hour of reperfusion and from sham operation group one hour after the operation. Electronic microscope technique was used to observe the ultrastructural changes of cortex.MAIN OUTCOME MEASURES: Ultrastructural changes of cortex.RESULTS: The neurons of cortex shrank to certain degree in the early stage of ischemic reperfusion(1 hour) . The glial cells were swollen with dissolved chromosome in nucleus and unclear nuclear membrane. The foot protrusions around blood vessel slightly swelled and separated from basement membrane. Mircro-tubes were partially dissolved.CONCLUSION: In early stage of reperfusion injury, the cortex neurons, glial cells, cellular framework and blood brain barrier already changed which suggested that the protective treatment such as reducing brain edema, protecting blood brain barrier should start as early as possible.关键词
脑缺血/再灌注损伤/神经元/超微结构分类
医药卫生引用本文复制引用
王涛,阚清,罗芳,赵继宗,孙异临..全脑缺血再灌注损伤大鼠早期脑皮质超微结构的变化[J].中国临床康复,2005,9(5):220-221,2.
