摘要
Abstract
Objective To investigate the polymorphism of 19 antigens of 8 rare red blood group systems in Shanghai clinical transfusion Han population in order to improve the capability of compatible infusion and reduce the transfusion reactions. Methods The genotypes were performed by polymerase chain reaction using sequence-specific primer(PCR-SSP).Results The gene frequencies of Diego,Dombrock,Duffy rare red blood group systems were Dia=0.035 1,Dib=0.964 9;Doa=0.061 4,Dob=0.938 6;Fya=0.964 9,Fyb=0.035 1,Fy=0;while the Kell,Yt,Scianna,L-W,Colton rare red blood groups allele frequencies distribution is monomorphic. There was a good fit to Hardy-weinberg equilibrium within each group.Conclusion There are great differences among the Diego,Dombrock,Duffy blood group gene frequencies in shanghai Han population.The rate of antigen mismatches in randomized clinical blood transfusion was 0.065 4,0.108 6,0.065 4 respectively.关键词
红细胞/稀有血型/基因分型/基因频率/PCR-SSPKey words
Red blood cell Rare blood group Gene typing Gene frequency PCR-SSP分类
医药卫生
