果树学报2005，Vol.22Issue(3)：220-223,4.
葛枣猕猴桃组织培养中的器官形成与植株再生
Organ formation and plant regeneration in vitro of Actinidia polygama
刘永立 1兰大伟 2毕静华 1陈超 1Takashi Harada1
作者信息
- 1. 浙江大学园艺系,农业部园艺植物生长发育与生物技术重点开放实验室,杭州,310029
- 2. Graduate School of Agriculture,Hokkaido University,Sapporo 060
- 折叠
摘要
Abstract
Petiole explants collected from Actinidiapolygama were cultured on BW media (a combined medium of half strengths of both broad-leafed tree medium and woody plant medium) containing 30 g/L sucrose and 8 g/L agar, and 12combinations of IBA (0, 0.01, 0.1 or 10 μmol/L) and CPPU (0, 0.1 or 1 μmol/L). Callus formation was promoted by increasing CPPU and IBA concentrations. Adventitious buds formed from the outer layer of callus that derived from the petiole explants from A. polygama. Shoot formation was increased with the increase of CPPU concentration and decreased with the increase of IBA concentration. When the shoots were transferred to BW medium supplemented with 1 μmol/L NAA alone,the intact shoots showed a 100% rooting rate after 40 days, and grew very healthily.关键词
葛枣猕猴桃/不定芽/CPPU/IBAKey words
Actinidiapolygama/Adventitious bud/Shoot/CPPU/IBA分类
农业科技引用本文复制引用
刘永立,兰大伟,毕静华,陈超,Takashi Harada..葛枣猕猴桃组织培养中的器官形成与植株再生[J].果树学报,2005,22(3):220-223,4.
