中国动脉硬化杂志2009，Vol.17Issue(3)：220-224,5.
急性冠状动脉综合征患者巨噬细胞表达过氧化体增殖物激活型受体γ及炎症相关因子的变化
Changes of Expression Levels of Peroxisome Proliferator-Activated Receptor-γ and Inflammatory Correlation Factors by Peripheral Blood Monocyte-Derived Macrophages in Patients with Acute Coronary Syndrome
摘要
Abstract
Aim To explore the changes of peroxisome proliferator-activated receptor-γ ( PPAR-γ), nuclear factor-κ (NF-κ) , metalloproteinnse-9 (MMP-9) and metalloproteinase inhibitor-1 (TIMP-1) expressed by peripheral blood monocyte-derived macrophages (MDM) in patients with acute coronary syndrome (ACS) and the relations among them. Methods 48 patients with ACS and 28 cases with stable angina pectoris (SAP) were admitted. A 20 mL fasting blood sample was obtained using a blood cell-separator tube. Peripheral blood mononuclear cells were isolated, and macrophage colony-stimulating factor was added to train them for MDM. After CD40 ligand stimulating, the concen-trations of MMP-9 and TIMP-1 in the supernate of MDM and the expression strength of PPAR-γ, MMP-9, TIMP-1 mRNA and NF-κ subunit P65 (NF-κ P65) by MDM were determined. Results In ACS group, the expression strength of PPAR-γmRNA was lower than the SAP group (0.24±0.04 vs 0.39±0.06;P<0.001), while the expression strength of NF-κ P65 was higher than the SAP group (0.42±0.06 vs 0.27±0.02 ;P<0.001), both the concentration of MMP-9 and TIMP-1 in the supemate of MDM were significantly higher than the SAP group (231.11±51.47 μg/L vs 126.06±13.2 μg/L, P <0.001 ;139.80±31.54μg/L vs 112.250 vs 12.68 μg/L, P <0.05), the expression strength of mRNA of MMP-9 and TIMP-1 were higher than the SA group (0.67±0.11 vs 0.24±0.05, P<0.001 ;0.42±0.09 vs 0.33±0.06, P<0.05). The expression strength of PPAR-γmBNA were negatively correlated to NF-κ P65 and the expression strength of MMP-9 (P<0.001), but not correlated to the expression strength of TIMP-1 (P>0.05);the expression strength of NF-κ P65 were positively correlated to the expression strength of MMP-9 and TIMP-1 (P<0.001). Con- clusion For MDM of patients with ACS, the expression strength of PPAR-γ was down-regulated, while the expression strength of NF-κ and MMP-9 was up-regulated. The PPAR-γ may regulate MMP-9 expression before transcribed by reg-ulating the activity of NF-κ. TIMP-1 expression may not be regulated by PPAB-γ.关键词
急性冠状动脉综合征/单核细胞源性巨噬细胞/过氧化体增殖物激活型受体γ/核因子κB/基质金属蛋白酶9/组织型基质金属蛋白酶抑制剂1Key words
Acute Coronary Syndrome/Monocytes Derived Macrophage/Peroxisome Proliferater-Activated Receptor/Nuclear Faetor-κ /Metalloproteinase-9 /Metalloproteinase Inhibitor-1分类
医药卫生引用本文复制引用
罗玉梅,姜德谦,万新红,刘显庆,郭洪波,陈朝霞,王合金,谢丽华..急性冠状动脉综合征患者巨噬细胞表达过氧化体增殖物激活型受体γ及炎症相关因子的变化[J].中国动脉硬化杂志,2009,17(3):220-224,5.基金项目
深圳市科技基金资助项目(JH200507120929A)和龙岗区科技基金资助项目(200514) （JH200507120929A）
