首页|期刊导航|高技术通讯（英文版）|Selection of anticoagulant solution to the hemolymph of Chlamys farreri by transmission electron microscropy and flow cytometry
高技术通讯（英文版）2007，Vol.13Issue(2)：221-224,4.
Selection of anticoagulant solution to the hemolymph of Chlamys farreri by transmission electron microscropy and flow cytometry
Selection of anticoagulant solution to the hemolymph of Chlamys farreri by transmission electron microscropy and flow cytometry
摘要
Abstract
Mixtures of hemolymph from Chlamys farreri with three different anticoagulant solutions were incubated for an hour in vitro , then the ultrastructural alterations of hemocytes were observed , and the aggregation rate was analyzed by using transmission electron microscropy and flow cytometry respectively. The results showed that Formula 3 (glucose 20. 8 g L-1; EDTA 20mM ; sodium chloride 20 g L-1 ; Tris-HCl 0.05M;pH 7. 4) was the desirable anticoagulant solution for C . Farreri hemocytes. Further phagocytosis assay showed that no obvious negative effect was given to the hemocyte phagocytic activity when using Formula 3 as the anticoagulant solution.关键词
hemolymph/anticoagulant solution/TEM/flow cytometry/Chlamys farreriKey words
hemolymph/anticoagulant solution/TEM/flow cytometry/Chlamys farreri分类
医药卫生引用本文复制引用
Chen Muyan,Yang Hongsheng,Shi Fangfang..Selection of anticoagulant solution to the hemolymph of Chlamys farreri by transmission electron microscropy and flow cytometry[J].高技术通讯（英文版）,2007,13(2):221-224,4.基金项目
Supported by the National Natural Science Foundation of China (No.30170742) and National Key Foundational Research Project of China (No.G1999012012). （No.30170742）
