摘要
Abstract
Time-correlated single photon counting (TCSPC) is based on the detection of single photons of a periodic light signal,the measurement of the detection time of the photons,and the build-up of the photon distribution versus the time in the signal period.The limitations of classic TCSPC were low count rate,long acquisition time,and the fact that the technique was intrinsically one-dimensional.We have developed an advanced TCSPC technique featuring multi-dimensional photon acquisition and a count rate close to the capability of currently available detectors.The technique is able to acquire photon distributions versus wavelength,spatial coordinates,the time on the ps scale,and a second time counting from the start of the experiment.Multi-dimensional TCSPC achieves a near-ideal counting efficiency and a time resolution limited only by the transit-time-spread of the detector.The technique is therefore exceptionally useful for biomedical spectroscopy.Typical applications include time-domain optical tomography,recording of transient phenomena in biological systems,fluorescence lifetime imaging microscopy,FRET experiments in living cells,and the investigation of dye-protein complexes by fluorescence correlation spectroscopy.We demonstrate the potential of the technique for a number of selected applications.关键词
时间相关单光子计数/多维探测/生物医学光谱学/荧光寿命成像显微术/荧光共振能量转移Key words
time-correlated single photon counting/multi-dimensional detection/biomedical spectroscopy/fluorescence lifetime imaging microscopy/fluorescence resonance energy transfer分类
信息技术与安全科学
