中德临床肿瘤学杂志(英文版)2008，Vol.7Issue(4)：223-226,4.
骨髓来源内皮前体细胞促进肿瘤新生血管生成
Bone marrow-originated endothelial progenitor cells contribute to neovasculature of tumor
摘要
Abstract
Objective:Neovascularization of tumor is a complex process.In this study,we aimed to reveal whether the bone marrow-originated endothelial progenitor calls (EPCs) contributed to neovasculature in tumor and the angiogenesis-associated factors,VEGF and B-FGF,enhanced this process.Methods:We had established a mouse model,which were deprived of bone marrow by radiation and transplanted with bone marrow of syngenetic GFP (Green Fluorescence Protein)-transgened mice,then implanted Lewis calls.Immunohistochemical and immunoflourensenca proved the EPCs location in tumors by indentifying colocalization of GFP expression in cells staining with endothelial progenitor cell markers,CD 133,ICAM-1,CD31.The growth statue and MVD of tumor was observed after injection of VEGF or B-FGF.ICAM-1 and VE-cadherin in tumor were detected by Western blot.Results:By immunohistochemical and immunoflourensence,we proved part of bone marrow precursors located in area of tumor angiogenesis and VEGF or B-FGF increased the MVD of tumor.In Western blot,it was found and VEGF or B-FGF up-regulate the expression of ICAM-1,VE-Cadherin.Conclusion:Bone marrow-derived endothelial progenitor cell seem to be recruited in neovasculature induced by tumor.VEGF and B-FGF are key regulators of this process.关键词
angiogenesis/endothelial progenitor calls/VEGF/B-FGFKey words
angiogenesis/endothelial progenitor calls/VEGF/B-FGF分类
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Yizhou Luo,Xi Wang,Ziheng Guo,Guanzheng Yu,Jiejun Wang..骨髓来源内皮前体细胞促进肿瘤新生血管生成[J].中德临床肿瘤学杂志(英文版),2008,7(4):223-226,4.基金项目
Supported by a grant from the National Key Project of Scientific and Technical Supporting Programs Funded by Ministry of Science & Technology of China (No.2006BA102A05). （No.2006BA102A05）
