国际眼科杂志2005，Vol.5Issue(2)：225-228,4.
缺氧时视网膜血管内皮细胞缺氧诱导因子-1α及其mRNA的时序性表达
Sequential changes of HIF-1αprotein and mRNA in hypoxic bovine retinal microvessel endothelial cells
邓爱军 1姜德咏1
作者信息
- 1. 410011,中国湖南省长沙市,中南大学湘雅二医院眼科
- 折叠
摘要
Abstract
● AIM: To investigate the sequential changes of HIF-1 α Protein and mRNA in hypoxic bovine retinal microvessel endothelial cells.● METHODS: The bovine retinal microvessel endothelial cells were cultured in normoxic and CoCl2-induced hypoxic conditions respectively. Expressions of HIF-1 α Protein were measured with immunohistochemical staining, and RT-PCR was used to determine the HIF-1 α mRNA.● RESULTS: HIF-1 α began to increase 1h after hypoxia,and reached the peak at 4h. After 16h, it declined significantly. Compared with the normoxic group, the expression of HIF-1 α protein in the hypoxic groups had significant difference (P＜0.01), and HIF-1 α mRNA expression was unchanged under hypoxia.● CONCLUSION: HIF-1 α participates in the hypoxic procedures in retinal microvessel endothelial cells, and hypoxia induce time-dependent changes of HIF-1 α protein expression, which is not modulated on the transcription level. Analysis of HIF-1 α expression revealed a temporal and spatial changes with regard to the hyperoxic repression, indicating that HIF-1 may play a major role in the development of retinopathy of prematurity and other ischemic retinal disorders such as diabetic retinopathy.关键词
缺氧诱导因子-1α/视网膜/血管内皮细胞Key words
HIF-1 α/retina/microvessel endothelial cells分类
医药卫生引用本文复制引用
邓爱军,姜德咏..缺氧时视网膜血管内皮细胞缺氧诱导因子-1α及其mRNA的时序性表达[J].国际眼科杂志,2005,5(2):225-228,4.
