中国临床康复2005，Vol.9Issue(3)：226-228,3.
微囊化转心房肽基因细胞分泌心房肽近日节律的体外研究
In vitro study of atrial natriuretic peptide circadian secreted by encapsulated atrial natriuretic peptide cDNA transfection cells
摘要
Abstract
AIM:To investigate atrial natriuretic peptide(ANP) circadian in the encapsulated human ANP(hANP) cDNA transfected cells,to alter the ANP circadian by artificial control to achieve the objective of effectively treat hypertension or congestive heart failure(CHF). METHODS:ANP cDNA was transfected into Chinese hamster ovary(CHO) cells,which were encapsulated in polycarprolactone(PCL) tubes.The longterm survival of transfected CHO cells and the levels of ANP secreted were detected.Circadian rhythm of ANP secreted by encapsulated transfected cells was also studied,which was regulated by melatonin. RESULTS:During culturing,the ANP level secreted by transfected CHO cells in 2 mL of culture medium within 24 hours could reach 210.3 ng/L in a 20 mm-long and 2 mm-diameter PCL tube.The section of ANP displayed a circadian variation:higher in daytime,but lower at night.The acrophase of circadian rhythm was 4:15 but could be shifted to 7:55 after melatonin management. CONCLUSION:ANP cDNA transfected CHO cells that encapsulated into PCL tube can secret ANP,which might be suitable for the future implantation into human body.Our research provides a new approach in the treatment of hypertension and CHF by ANP.关键词
受体/心钠素/时间生物学/节律性分类
医药卫生引用本文复制引用
付泳航,李若冰,陈立国,肖静,郭慧玲,郭丽,王正荣,万朝敏..微囊化转心房肽基因细胞分泌心房肽近日节律的体外研究[J].中国临床康复,2005,9(3):226-228,3.基金项目
本课题部分由国家自然科学基金资助(30070278 （）
39970275) （）
