中国临床康复2005，Vol.9Issue(11)：226-228,3.
基质金属蛋白酶3,12基因多态性与汉族人群急性冠状动脉综合征危险性的关系
Association of matrix metalloproteinase-3 and -12 genes polymorphism with acute coronary syndrome in Chinese Han population
熊鹰 1魏晓艳 1杨秀兰 1王玲 2何春燕 3周新4
作者信息
- 1. 武警湖北总队医院急诊科,湖北省,武汉市,430061
- 2. 华中科技大学梨园医院内科
- 3. 武汉大学医学院生化与分子生物学系
- 4. 武汉大学中南医院检验系,湖北省,武汉市,430071
- 折叠
摘要
Abstract
BACKGROUND: Acute coronary syndrome(ACS) are a result of the rupture of unstable atherosclerotic plaques and thrombosis. Matrix metalloprotei nases (MMPs) are believed to play an important role in vascular remodeling and plaque instability by degrading the extracellular matrix.OBJECTIVE: To investigate the possible impact of polymorphisms in matrix metalloproteinase-3 and -12 genes on the risk of ACS in Chinese Han population.DESIGN: A case-control study based on ACS patients as subjects and healthy population as the control group.SETTING: Department of clinical tests of a university hospital and the department of biochemistry and molecular biology of a medical college PARTICIPANTS: A total of 103 patients with ACS(outpatients and inpatients received and hospitalized from March 2002 to December 2003 at the People' s Armed Police Hubei General Hospital,Zhongnan Hospital of Wuhan University and Liyuan Hospital of Huazhong University of Science and Technology) were involved and were divided into ACS group and the normal control group. For the ACS group,there were 73 males and 30 females with an average age of(53±10) years,including 47 cases of acute cardiac infarction and 56 cases of unstable cardiac angina. For the normal control group,there were 100 healthy subjects as controls,including 67 males and 33 females with an average age of(52±12) years. All the subjects were Chinese Han Population in Hubei Province,and they were voluntary to participate in the study.METHODS: Polymerase chain reaction(PCR) and restriction fragment length polymorphism(RFLP) techniques were used to examine the MMP-3-1612 5A/6A and MMP-12 -82 A/G gene polymorphisms respectively.MAIN OUTCOME MEASURES: The genotypes of and the allele frequencies in the patients and the individuals in the control group.RESULTS: The prevalence of 5A/6A +5A/5A genotype(41.7% vs 24%,x2 =6. 447,P<0.05) and5A allele (21.8% vs 13%,χ2 =4. 906,P<0.05) of MMP-3 gene were significantly more frequent in the ACS group than in the control subjects. The odds ratio of the 5A/6A +5A/5A was 2. 269(95% CI,1.241 to 4. 149,P<0.01). No significant difference was detected between the patients and the control group in genotype and allele distribution of the MMP-12 -82 A/G gene polymorphism(P>0.05).CONCLUSION: These findings indicate that the 5A/6A polymorphism of MMP-3 gene is associated with the risk of ACS,and might play an important role in the development of coronary atherosclerosis..This conclusion provides experimental data for gene study of the coronary disease and primary intervention for rehabilitation.关键词
冠状动脉疾病/金属蛋白酶类/多态现象(遗传学)/聚合酶链反应分类
医药卫生引用本文复制引用
熊鹰,魏晓艳,杨秀兰,王玲,何春燕,周新..基质金属蛋白酶3,12基因多态性与汉族人群急性冠状动脉综合征危险性的关系[J].中国临床康复,2005,9(11):226-228,3.
