植物学通报2001,Vol.18Issue(2):226-230,5.
水稻种子萌发过程中α-淀粉酶
与萌发速率关系的分析
Analysis of the Relationship between α_Amylase and Germinating
Rate of Rice Seeds during the Process of Seed Germination
摘要
Abstract
The change of α-amylase activity and isoenzyme in embryos of Nongken 58F, Nongken 58S and Nongken 58 were analyzed by assay of reducing sugars with 3,5-dinitrosalicylic acid(DNS) and acrylamide gel electrophoresis technique under the different germinating time. The α-amylase activity in the embryos of both Nongken 58F and Nongken 58S were higher than that of Nongken 58 from 0 to 36 h germinating time. Rice seed embryos had 5 isoenzyme bands(A1,A2,A3,A4,A5). A2,A4 and A5 were present in the embryos of Nongken 58F, Nongken 58S and Nongken 58. A3 was specific for both Nongken 58F and Nongken 58S. A1 was specific for the embryos of Nongken 58. These results suggested a higher α-amylase activity be necessary for promoting fast germination of rice seeds, α-amylase isoenzyme A1 and A3 may play an important role in regulation of germination speed.关键词
水稻/种子萌发/α_淀粉酶分类
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赵玉锦,王台..水稻种子萌发过程中α-淀粉酶
与萌发速率关系的分析[J].植物学通报,2001,18(2):226-230,5.