中国药房2001，Vol.12Issue(4)：227-228,2.
高效液相色谱法测定人血清中5-氟尿嘧啶的浓度
Determination of Fluorouracil in Human Serum With HPLC
摘要
Abstract
OBJECTIVE: To determine the content of 5-Fu in human serum.METHODS: Serum protein was precipitated with trichloroacetic acid .HPLC was used for determination of 5-Fu .The column was Shimpack CLC C18(150 × 6mm, 5μm)with YWG for protection.The mobile phase was 0.25% KH2PO4-acetonitrile(98: 2, pH7.0) .The flow rate was 1ml/min.The detection wavelength was 265nm.Two standard curves were made in the concentrations of 0.195～6.250μg/ ml and 6.25～200.00μg/ml, respectively.RESULTS: The calibration curves revealed linearity in the range of 0.195～200μg/ml.The with-in-day recoveries and RSDs of 5-Fu at 0.8, 4, 20 and 100μg/ ml concentration points were 104. 88%, 1.95%; 104.58%,1.38%; 101.40%, 0.39%and 99.14%, 0.37%, and the between-day recoveries and RSDs of 5-Fu were 105.12%, 2.02%;106.30 %, 0.78 %; 100.60 %, 0.65 % and 99.38 %, 0.92 % respectively. CONCLUSION: This method is rapid, accurate and suitable for pharmacokinetical study and conventional monitoring of 5-Fu.关键词
5-氟尿嘧啶/高效液相色谱/血药浓度分类
医药卫生引用本文复制引用
姚水宝,杨水新..高效液相色谱法测定人血清中5-氟尿嘧啶的浓度[J].中国药房,2001,12(4):227-228,2.
