农药学学报2006，Vol.8Issue(3)：227-232,6.
绿磺隆、醚苯磺隆及其体外代谢物对乙酰乳酸合成酶的离体抑制作用
Inhibition of Acetolactate Synthase Activity by Chlorsulfuron, Triasulfuron and Their Metabolites in vitro
摘要
Abstract
The metabolism of two sulfonylurea herbicides chlorsulfuron and triasulfuron was investigated by using wheat cytochrome P450 cDNA(CYP71C6v1) which expressed in yeast. The results showed that chlorsulfuron and triasulfuron were hydroxylated at 5-position on the phenyl ring. The difference in IC50 of parent compound and 5-hydroxy metabolites to acetolactate synthase (ALS) was small. However, their metabolites injured neither wheat nor bean, when applied as post-emergence treatment.The IC50 of chlorsulfuron and its metabolites to wheat ALS were 7.1 × 10-9 and 7.9 × 10-9 mol/L,respectively,and to bean ALS were 3. 6 × 10-9 and 4. 1 × 10-9 mol/L, respectively. The IC50 of triasulfuron and its metabolites to wheat ALS were 4.6 × 10 -9 and 5.3 × 10-9 mol/L,respectively, and to bean ALS were 4.7 × 10-9 and 4.9 × 10-9 mol/L,respectively. These results suggested that proper groups at 5-position on phenyl ring of sulfonylurea molecule might possess high herbicidal activity.关键词
细胞色素P450/绿磺隆/醚苯磺隆/代谢产物/乙酰乳酸合成酶/抑制作用Key words
cytochrome P450/chlorsulfuron/triasulfuron/metabolite/acetolactate synthase/inhibition分类
农业科技引用本文复制引用
王相晶,向文胜,郑永权,姜玲..绿磺隆、醚苯磺隆及其体外代谢物对乙酰乳酸合成酶的离体抑制作用[J].农药学学报,2006,8(3):227-232,6.基金项目
Supported by the National Natural Science Foundation of China (No. 30000113 and 30270888 ). （No. 30000113 and 30270888 ）
