中国现代医学杂志2008，Vol.18Issue(16)：2278-2282,2286,6.
MMP-9和TIMP-1在高氧致CLD新生大鼠肺组织的动态变化及其对Ⅳ型胶原的影响
Dynamic expression and effects of MMP-9 and TIMP-1 on type Ⅳ collagen in lung tissue of neonatal rats with hyperoxia-induced CLD
摘要
Abstract
[Objective]To investigate the dynamic changes of matrix metalloproteinase-9 (MMP-9) and tissue inhibitors of metalloproteinase-1 (TIMP-1) in lung of neonatal rats after inhaling high concentration of oxygen (hyperoxia), and the effects on type Ⅳ collagen remodeling. [Methods]Full-term newbem rats were continuously exposed to oxygen (0.90～0.95) or room air (0.2102) within 12 hours after birth. On day 1, 3, 7, 14 and 21, the contents of type Ⅳ collagen in lungs were detected by enzyme linked immunosorbent assay (ELISA), the changes of MMP-9 and TIMP-1 expression were measured by immunohistochemistry. [Results]The protein content of type Ⅳ collagen in hyperoxia group increased significantly on the 14th day [(24.78±5.42) vs (14.90±2.44), P<0.05]and the 21st day [(40.27±1.94) vs (26.13±1.94), P<0.01]compared to that in control group, the expression of MMP-9 increased on the 3rd day in hyperoxia groups, the average values of protein were (126.23±6.95) vs (130.38±7.36) (P<0.05), while no difference was seen on other days, higher values of TIMP-1 protein expression were seen on the 3rd day [(126.22±6.49) vs (129.49±4.75)]and the 7th day [(119.70±7.33) vs (124.99±6.83)](P<0.05), which increased significantly compared with the control group on the 14th day [(112.35±10.29) vs (120.08±7.77)]and the 21st day [(109.19±10.56) vs (118.22±6.32)](P<0.01). [Conclusion]With prolonged hyperoxia, the balance of MMP-9/ TIMP-1 can not been kept, type Ⅳcollagen breakdown is not balanced, which maybe make the basement membrane damage in early stage and lead to type Ⅳ collagen deposition and lung fibrosis in late stage.关键词
新生大鼠/高氧/Ⅳ型胶原/MMP-9/TIMP-1Key words
neonatal rat/hyperoxia/type Ⅳ collagen/matrix metalloproteinase-9/tissue inhibitors of metalloproteinase-1分类
医药卫生引用本文复制引用
刘雪雁,徐刚,薛辛东..MMP-9和TIMP-1在高氧致CLD新生大鼠肺组织的动态变化及其对Ⅳ型胶原的影响[J].中国现代医学杂志,2008,18(16):2278-2282,2286,6.基金项目
Supported by National Science Foundation of China (No. 30440056) （No. 30440056）
