中德临床肿瘤学杂志（英文版）2007，Vol.6Issue(3)：228-231,4.
外源人核受体hLRH-1在结肠腺癌细胞SW480中表达的生物学效应及其分子机理初探
Biological effect of expression of exogenous human nuclear receptor hLRH-1 in SW480 cells and its molecular mechanism
摘要
Abstract
Objective:To explore the possible biological function of human nuclear receptor hLRH-1 in tumorigenesis and progress of colon cancer.Methods:Plasmids pcDNA3-hLRH-1 were introduced into SW480 ceils via lipofectamine.The expression of mRNA and protein of exogenous hLRH-1 were detected by RT-PCR and western blotting.respectively.MTT assay was carried out to survey the proliferation of SW80 cells with overexpression of hLRH-1.Meanwhile.the expression of proliferation-related genes cyclin E1 and cyclin D1.and apoptosis-related genes PTEN and Rb1.were analyzed by realtime RT-PCR.Result:The proliferation of SW480 cells was promoted under the condition of overexpression of hLRH-1.The expression of cyclin E1 was up-regulated significantly,while that of PTEN and Rb1 were down-regulated jn SW480 cells with overexpressed hLRH-1.Conclusion:The expression of exogenous hLRH-1 in SW480 cells induced the proliferation resulting form up-regulation of cyclin E1.as well as participated in the regulation of apoptosis via influencing the expression of PTEN and Rb1.关键词
nuclear receptor hLRH-1:colon cancer/expression of exogenous gene/biological effectKey words
nuclear receptor hLRH-1:colon cancer/expression of exogenous gene/biological effect分类
医药卫生引用本文复制引用
Shuiliang Wang,Yingmeng Zheng,Fenghua Lan..外源人核受体hLRH-1在结肠腺癌细胞SW480中表达的生物学效应及其分子机理初探[J].中德临床肿瘤学杂志（英文版）,2007,6(3):228-231,4.基金项目
Supported by grants from the Young Scientific and Technical lnnovation Foundation of Fujian Province(No.2004J067),and Foundation of Fuzhou General Hospital(No.200638). （No.2004J067）
