湖南农业大学学报（自然科学版）2009，Vol.35Issue(3)：229-232,4.
SSR标记技术在甘蓝型油菜杂交种纯度鉴定中的应用
Application of SSR molecular marker technique on purity identification of hybrid in rapeseed
摘要
Abstract
Purity identification of seed production samples of three hybrid rapeseed(Brassca napus L.) varieties,Fengyou701,Fengyou730 and Xiangzayou4,was conducted by simplified SSR molecular marker technique,and the identification results on premature pods samples and mature seeds samples by two identification methods,SSR and planting in field,were compared. And then,SSR identification results and the fertility performance of all plants came from matured seeds of Fengyou701 were contrasted one by one. These results showed that: There was a big difference in the size of the amplification segment between the 3 varieties from both fertility and restore lines by primer Na10-D09 selected from 38 SSR primer pairs.Comparative analysis indicated that SSR molecular marker technique produced a higher veracity in purity identification.关键词
油菜/杂交种/纯度鉴定/微卫星Key words
rapeseed/hybrid/purity identification/SSR分类
农业科技引用本文复制引用
曲亮,陈卫江,李莓,王同华..SSR标记技术在甘蓝型油菜杂交种纯度鉴定中的应用[J].湖南农业大学学报（自然科学版）,2009,35(3):229-232,4.基金项目
国家科技支撑计划项目(2006BAD01A04) （2006BAD01A04）
