癌症2001，Vol.20Issue(3)：229-235,7.
垂体瘤发病相关基因的大规模筛选及鉴定
Large-Scale Screening for Genes Related to Pathogenesis of Pituitary Carcinoma
宋怀东 1彭永德 1胡仁明 1陈家伦 1黄秋花 2周隽 2吴昕彦 2张庆华 2任双喜 3付刚3
作者信息
- 1. 第二医科大学附属瑞金医院,上海市内分泌研究所,
- 2. 上海血液学研究所,
- 3. 国家人类基因组南方研究中心,
- 折叠
摘要
Abstract
Objective： The aim of this study was to investigate the method of large-scale screening for genes differentially expressed in nonfunctional pituitary tumor and normal pituitary. Methods： cDNA libraries of nonfunctional pituitary tumor (NFPT) and human normal pituitary were constructed. Large-scale sequencing, bioinformatics and semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) were used. Significant difference of known genes expression in 2 tissues were analyzed by statistical software offered by Audic S and Claverie JM (http： //igs-server.cnrs-mrs.fr/). Results： A total of 1253 and 7222 ESTs with good quality were obtained from the tissues of NFPT and normal pituitary, respectively. Two hundred known genes were found in NFPT. Among them, 38 genes creditability of significant difference expression in the 2 tissues were over 0.99, and 130 were over 0.95. Out of the 130 differentially expressed genes in the 2 tissues, 17 genes were associated with cell differentiation and development (G2). 6 differentially expressed G2 category genes were selected and the liability of the expression significant difference in the 2 tissues was confirmed by semi-quantitative RT-PCR. Out of them, 4 were confirmed differentially expressed in the 2 tissues. Conclusion： MEIS2, SMT3C, C1D, and BUB3 genes associated with cell differentiation and development (G2) were expressed more in nonfunctional pituitary tumor than in normal pituitary. They may play a role in the pathogenesis of nonfunctional pituitary carcinoma.关键词
垂体瘤/基因表达谱/生物信息学/半定量RT-PCR分类
医药卫生引用本文复制引用
宋怀东,彭永德,胡仁明,陈家伦,黄秋花,周隽,吴昕彦,张庆华,任双喜,付刚..垂体瘤发病相关基因的大规模筛选及鉴定[J].癌症,2001,20(3):229-235,7.
