生命科学研究2000，Vol.4Issue(3)：231-236,6.
子房注射法与农杆菌介导法转化甘蓝型油菜的比较研究
Comparative Studies on the Genetic Transformation of Brassica napus by Using Agrobacterium-mediated and Ovary-injection Techniques
摘要
Abstract
Kanamycin-resistant Brassica napus cv.Xiangyou 13 were obtained by agrobac-terium tumefaciens-mediated genetic transformation at the highest frequency of 8.3% when cotyledonary petioles were immersed into the agrobacterium suspension for 3～5 min and co-cultured for 60～72 h, whereas by ovary-injecting genetic transformation it was succecded at the highest frequency of 12.8% when 0.5～1.5 μg of exogenous DNA was injected into the middle of an ovary from 20th hour to 30th hour after pollination.Southern blotting analysis of the total DNA extracted from transgenic plants indicated that Bt insecticidal protein gene had been integrated into the genome of oilseed rape．Transgenic plants were obtained by agrobacterium tumefaciens-mediated and ovary-injected at frequency of 1.18%，2.66% respectively. Ovary injection was practical and efficient for oilseed rape transformation.关键词
甘蓝型油菜/遗传转化/Bt杀虫蛋白基因Key words
Brassica napus/genetic transformation/Bt insecticidal protein gene分类
农业科技引用本文复制引用
林良斌,官春云,李恂,周小云,何业华..子房注射法与农杆菌介导法转化甘蓝型油菜的比较研究[J].生命科学研究,2000,4(3):231-236,6.基金项目
湖南省科委基金项目 （）
